Ubiquitin metabolism in HeLa cells starved of amino acids
نویسندگان
چکیده
منابع مشابه
Reutilization of amino acids in protein synthesis in HeLa cells.
On the assumption that all amino acids entering protein must pass through the intracellular pool of free amino acids, reutilization of leucine, lysine, arginine, and alanine was determined by pulse labeling of HeLa cell protein, followed by a chase in unlabeled medium and measurement of the specific activities of the intracellular free pool and protein amino acids. The half-lives of the amino a...
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Parameters of branched-chain amino acids (BCAA; leucine, isoleucine and valine) and protein metabolism were evaluated using L-[1-(14)C]leucine and alpha-keto[1-(14)C]isocaproate (KIC) in the whole body and in isolated perfused liver (IPL) of rats fed ad libitum or starved for 3 days. Starvation caused a significant increase in plasma BCAA levels and a decrease in leucine appearance from proteol...
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Methods have been developed to study cellular Na, K, and Cl concentrations in HeLa cells. Cell [Na] and [K] are functions of the age of the culture. As the culture grows [K], expressed in mmols/liter cell H(2)O, rises from an initial value of 121 to a peak of 206 at about 4 days, and thereafter falls until it has almost returned to the initial value by the 9th day. [Na] falls as [K] rises, but ...
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It was shown by Seth and Luck (1) that the intestinal absorption of glycine and alanine by rabbits was followed by pronounced aminoacidemia. Other amino acids when administered in similar amounts provoked smaller increases in the amino nitrogen content of the blood. Glutamic acid and aspartic acid were absorbed with but a slight aminoacidemia. The question then arose as to whether these strikin...
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Radioiodinated ubiquitin was introduced into HeLa cells by erythrocyte-mediated microinjection. Subsequent electrophoretic analyses revealed that the injected ubiquitin molecules were rapidly conjugated to HeLa proteins. At equilibrium, 10% of the injected ubiquitin was conjugated to histones and 40% was distributed among conjugates of higher molecular weight. Although the remaining ubiquitin m...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1992
ISSN: 0014-5793
DOI: 10.1016/0014-5793(92)80757-8